【摘要】 对比甲醇-盐酸混合物及硫酸处理对场效应器件(Field-effect device,FED)电化学测试性能的影响。实验结果表明,FED表面经过两种方式处理,其电化学阻抗测试特性发生变化,表现在阻抗的虚部值(用Zq表示)随加在FED器件与参比电极之间的电压的变化特性(用Zq-V曲线表示)发生改变。经过硫酸处理的器件的Zq-V曲线相对于未处理器件有微小偏移;而甲醇-盐酸体系处理后曲线在耗尽层区的斜率有所下降,表明这种处理对器件测试性能有负向的影响。经硅烷化固定在表面的氨基采用荧光标记,荧光测试结果表明,两种表面处理方式对硅烷化效果影响不大。进一步通过共价连接在硅烷化FED表面引入探针DNA分子,制备基于FED的DNA传感器。与目标DNA分子杂交反应后,实验表明,硫酸处理的测试结果要好于甲醇-盐酸的处理方法。更多还原

【Abstract】 The surface modification was carried out employing sulfuric acid and methanol-HCl solution respectively.The test characteristics were illustrated by the measurement of the out-phase impedance(referred to as Zq) vs bias voltage imposed between the FED and the reference electrode.The Zq-V curves are different after different surface modifications.With the sulfuric acid treatment,the Zq-V curve changes little,while the treatment by the methanol-HCl solution will cause the decrease of the sensitivity of the FED.The silanization and fluorescin-labeling of the surface after these two kinds of treatment reveal that the treatments cause little influence on the silanization effect of the surface.The FED-based DNA sensors were prepared after the surface modification of the FED.The methanol-HCl treatment will make the readout of the DNA sensor decreased compared with the sulfuric acid treatment.更多还原