【摘要】 目的探讨冬凌草甲素对人白血病细胞株HL-60细胞生长抑制作用及其机制。方法收集经不同浓度冬凌草甲素干预的HL-60细胞,制成细胞涂片,光学显微镜下观察细胞形态。采用MTT比色法观察不同浓度冬凌草甲素对HL-60细胞增殖的抑制作用,通过凋亡试剂盒Annexin V-FITC标记,流式细胞仪检测细胞凋亡率,并以RT-PCR检测凋亡抑制基因Survivin的表达。结果冬凌草甲素在10~25μmol/L范围内可抑制HL-60细胞的生长,且呈时间和浓度依赖性。并可诱导HL-60细胞发生凋亡,表现出特异性的凋亡形态学改变。流式细胞仪分析显示,冬凌草甲素处理后凋亡细胞比例增多,且随着药物浓度的增加而逐渐增加。RT-PCR检测结果显示,20μmol/L冬凌草甲素作用后,与对照组相比,HL-60细胞Survivin mRNA水平下降,且随时间延长表现得更为明显。结论冬凌草甲素能抑制人白血病细胞株HL-60细胞的生长增殖,并可诱导其发生凋亡,其作用机制可能与凋亡抑制基因Survivin的下调有关。更多还原

【Abstract】 Objective To investigate the anti-proliferation effect of rebescensine A on leukemia HL-60 cells and its mechanism.Methods HL-60 cells in culture medium in vitro were given different concentrations of rebescensine A.Morphological changes were observed in cell smears.MTT assay was used to observe the growth inhibition of HL-60 cells;Annexin V-FITC and flow cytometry were used to examine cell apoptosis rate.RT-PCR was used to observe the expression of Survivin.Results Rebescensine A(10-25 μmo1/L) could inhibit the growth of HL-60 cells and induce apoptosis significantly in a time-and dose-dependent manner.Marked morphological changes of cell apoptosis were observed.Apoptotic cell count increased along with increasing concentrations of rebescensine A.The down-regulated expression of Survivin mRNA was showed by RT-PCR and changed more obviously with prolongation in HL-60 cells treated by rebescensine A 20 μmol/L.Conclusion Rebescensine A could inhibit proliferation and induce apoptosis of HL-60 cells in vitro.One of the mechanisms may be the down-regulated expression of survivin mRNA.更多还原