【摘要】 目的:初步探索人羊膜基质细胞(hAMC)作为骨组织工程种子细胞的潜力。方法:分离培养hAMC,将第3代hAMC在含有地塞米松(0.1μmol/L)、维生素C(50mg/L)和β-磷酸甘油钠(10mmol/L)的DMEM培养液中进行成骨诱导培养1周,行茜素红染色,计数钙化结节形成的数量,并进行免疫荧光细胞化学染色以检测Ⅰ型胶原和碱性磷酸酶的表达。利用免疫组织(细胞)化学染色检测羊膜及hAMC中FasL的表达。结果:hAMC经过成骨诱导后,可见明显钙化结节形成,平均每孔18个,钙化结节处细胞Ⅰ型胶原和碱性磷酸酶表达阳性。人羊膜组织中细胞及体外培养的hAMC均可检测到FasL的阳性表达。结论:hAMC有潜力成为一种比较理想的骨组织工程的种子细胞。更多还原

【Abstract】 Objective:To investigate the potential of human amniotic mesenchymal cells(hAMC) serving as seeding cells in bone tissue engineering.Methods:hAMC were isolated and cultured.The third passage of hAMC was cultured in osteogenic induced media [DMEM supplemented with 10%(v/v) FBS,0.1 μmol/L dexamethasone,50 mg/L ascorbic acid and 10 mmol/L β-glycerophosphate] for one week.Calcified nodules were shown by alizarin red staining and counted under light microscope.Immunofluorescence cytochemical staining was used to detect collagenⅠ(COL Ⅰ) and alkaline phosphatase(ALP).Expression of FasL was examined in the amnion and hAMC by immunohistochemistry or immunocytochemistry.Results:After osteoblast differentiation,calcified nodules were formed,on the average 18 per well.hAMC in calcified nodules showed positive expression of COL Ⅰ and ALP.FasL was detected positive both in cells contained in amnion and in hAMC.Conclusion:hAMC are potential ideal candidates for seeding cells in bone tissue engineering.更多还原