【摘要】 目的:探讨激活素A(ActA)及其结合蛋白follistatin(FS)在小鼠胰腺损伤过程中的表达,为胰腺损伤后组织修复与重建奠定基础。方法:雄性ICR小鼠21只,随机分为对照组(n=6)与实验组(n=15)。应用一次性大剂量腹腔注射链脲菌素(STZ)制备小鼠胰岛损伤模型,免疫组织化学染色法检测ActA和FS蛋白表达,逆转录聚合酶链反应(RT-PCR)法检测ActA和FS mRNA相对转录水平。结果:免疫组织化学染色显示,ActA主要分布在正常小鼠胰岛细胞,腺泡细胞也有表达;FS仅表达在小鼠胰岛细胞,在腺泡细胞不表达。RT-PCR检测结果显示,ActA和FS mRNA均在正常小鼠胰腺组织表达,与对照组比较,实验组小鼠Act mRNA表达随着诱导时间的延长表达量进行性减少(P<0.01),而FS mRNA在诱导的第7天表达明显增加,第14和21天下降(P<0.01)。结论:ActA及FS主要表达在小鼠胰岛组织,在STZ诱导胰腺损伤过程中Act-FS系统表达失平衡,可能与糖尿病的发生、发展有关。更多还原

【Abstract】 Objective To investigate the expressions of activin A (Act A) and its binding protein follistatin(FS) in mice with pancreatic injury and lay a fundation for tissue repair and reestablishment after pancreas injury.Methods Twenty one male ICR mices were randomly divided into two groups :experimental group(n=15)and control group(n= 6),Mice were treated intraperitoneally with streptozotocin (STZ) to induce pancreatic injury.The expressions of Act A and FS protein were detected by immunohistochemistry,and the expressions of activin A and follistatin mRNA were examined by RT-PCR.Results In normal mice,Act A protein mainly distributed in islet cells,and also distributed in pancreatic acinar cells;FS protein only expressed in islet cells and not in acinar cells.Act A and FS mRNA expressed in pancreatic tissues.Compared with control group,the expression of Act A mRNA in mice in experimental group decreased gradually(P<0.01),the expression of FS mRNA increased at the 7th day after injury,then decreased gradually at the 14th and 21st day (P<0.01).Conclusion Act A and its binding protein FS mainly express in mouse islet tissues.The unbalance of activin Act-FS system in streptozotocin-induced pancreatic injury may be involved in the pathogenesis of diabetes mellitus.更多还原