【摘要】 目的:探讨姜黄素(Cur)对中波紫外线(UVB)照射后人类角质形成细胞(HaCaT)氧化致癌的预防作用及其机制。方法:细胞经UVB照射后,立即加入浓度分别为1.25、2.50和5.00mg.L-1的Cur继续培养,用酶化学法测定氧化和抗氧化物含量;由罗丹明123和Fluo-3/AM荧光强度计算细胞Δψ和[Ca2+]i,用流式细胞术检测凋亡相关因子蛋白表达。结果:与对照组比较,UVB照射组HaCaT细胞Δψ、SOD和GSH-Px含量及Bcl-2蛋白表达均显著下降(P<0.01),而细胞[Ca2+]i、ROS、NO和MDA含量以及caspase-3、cytochromc和Bax蛋白表达均明显升高(P<0.01);与UVB照射组比较,UVB照射后加入Cur各组细胞Δψ和Bcl-2蛋白表达以及2.50和5.00mg.L-1组的SOD和GSH-Px含量均明显升高(P<0.01),而各加药组的ROS、NO和MDA含量以及caspase-3、cytochromc和Bax蛋白表达和2.50和5.00mg.L-1组的[Ca2+]i均显著降低(P<0.01),Bcl-2/Bax比值为0.58～2.50,并显示Cur剂量-效应关系。结论:Cur可能通过提高线粒体膜电位和抗氧化酶活性,降低[Ca2+]i、氧化物含量及凋亡相关诱导因子蛋白表达,从而达到其抗UVB辐射细胞致癌的预防作用。更多还原

【Abstract】 Objective To study the preventive effect of curcumin (Cur) on ultraviolet B (UVB) oxidation carcinogenesis in HaCaT cells and its mechanisms. Methods 1.25,2.50 and 5.00 mg·L-1 Cur were added into HaCaT cells irradiated by UVB and the cells were cultivated continually.The contents of oxides and antioxides were determined with zymochemistry method;the Δψ potential and the [Ca2+]i were measured with Rhodamine 123 and Fluo-3/AM fluorescence.The protein expressions of apoptotic correlating factors were examined by flow cytometry. Results Compared with control group,the Δψ of the HaCaT cells ,the contents of SOD and GSH-Px and the expression of Bcl-2 protein in UVB irradiation groups decreased significantly after UVB irradiation (P<0.01);while the [Ca2+]i,the contents of ROS,NO and MDA,and the protein expressions of caspase-3,cytochrome c and Bax increased significantly (P<0.01).After Cur was added into the cells irradiated with UVB , the Δψ and the expression of Bcl-2 in every Cur group increased significantly,the contents of SOD and GSH-Px in 2.50 and 5.00 mg·L-1 Cur groups increased significantly(P<0.01),while the contents of ROS,NO and MDA and the protein expressions of caspase-3,cytochrom c and Bax in every Cur group and the [Ca2+]i in 2.50 and 5.00 mg·L-1 Cur groups decreased significantly compared with UVB irradiation group(P<0.01).The ratio of Bcl-2/Bax varied within 0.58-2.50 and Cur showed a pattern of dose-effect.Conclusion Cur could increase the Δψ and the antioxidase activity and decrease the [Ca2+]i,the contents of oxides and the protein expressions of induced apoptotic correlating factors. Cur has preventive effect on cancinogenesis of the cells irradiated by UVB.更多还原