【摘要】 目的:提取强力天麻杜仲胶囊(QLTM)的活性成分,检测其药物活性及其对巨噬细胞的调节作用。方法:随机将小鼠分为对照组(生理盐水组)、QLTM胶囊组(阳性药物组)及QLTM活性成分高、中、低剂量提取物组,连续灌胃14 d,通过热板致痛法观察小鼠首次舔后足时间和1 min内的舔后足次数,通过醋酸致痛法观察小鼠首次扭体时间和15 min内的扭体次数,通过腹腔巨噬细胞吞噬鸡红细胞实验检测巨噬细胞活性。结果:在热板致痛实验中,高、中剂量提取物组小鼠首次舔后足时间延长,1 min内的舔后足次数减少(P<0.05或P<0.01);在醋酸致痛实验中,高、中剂量提取物组小鼠首次扭体时间延长,15 min内的扭体次数减少(P<0.05);高、中剂量提取物组及QLTM胶囊组小鼠巨噬细胞吞噬鸡红细胞百分率均降低,但中剂量组低于对照组(P<0.05)。结论:QLTM活性成分具有镇痛和抑制巨噬细胞活性作用。更多还原

【Abstract】 Objective To prepare active constituent from Qianglitianmaduzhong capsule(QLTM) and observe the analgesic effect of active constituent on mice and regulatory effect on macrophages.Methods Mice were randomly divided into five groups: negative control group(normal saline group),QLTM capsule group(positive control group),active constituent groups with high,middle,and low doses.The mice ware administered by oral gavage every day for 14 d.In hot-plate test,the beginning time and times of licking hind paws within 1 min of mice were observed.In acetic acid-induced writhing test,the beginning time and writhing times within 15 min of mice were observed.The phagocytic ability of peritoneal macrophage was evaluated with chicken red blood cell phagocytosis assay.Results In hot-plate test,the active constituent with high and middle doses prolonged the beginning time of licking hind paws and reduced times of licking hind paws within 1 min(P<0.05 or P<0.01),compared with negative control group.In acetic acid-induced writhing test,the active constituent with high and middle doses prolonged the beginning time of acetic acid-reduced writhing and reduced writhing times within 15 min(P<0.05).Both active constituent and QLTM inhibited the phagocytic ability of mouse peritoneal macrophages,especially in middle-dose group of active constituent,statistically significant difference was observed compared with negative control group(P<0.05).Conclusion The active constituent from QLTM has analgesic effect on mice and can inhibit activation of macrophages.更多还原