【摘要】 目的探讨肺癌患者血清及外周血单个核细胞中血小板源性生长因子-BB(PDGF-BB)的表达水平及其意义。方法采用酶联免疫吸附试验(ELISA)检测30名肺癌患者和20名正常对照者外周血血清中PDGF-BB的水平,用半定量逆转录-聚合酶链反应(RT-PCR)方法检测受试者外周血单个核细胞(PBMCs)中PDGF-B链mRNA的表达水平。结果肺癌患者和正常对照者血清PDGF-BB水平分别为(389.64±36.42)、(28.36±8.35)pg/ml,外周血单个核细胞PDGF-B链mRNA的表达水平分别为(0.9287±0.0942)、(0.2275±0.0384)灰度比值,比较差异有统计学的意义(P<0.01)。结论检测血清PDGF-BB及PBMCs中PDGF-B链mRNA表达水平可能对肺癌诊断具有一定临床指导意义。更多还原

【Abstract】 Objective To investigate the expression levels of platelet-derived growth factor-BB(PDGF-BB) and its role in the pathogenesis of the patients with lung cancer. Methods The levels of serum PDGF-BB in 30 patients with lung cancer and 20 normal controls were measured by enzyme linked immunosorbent assay( ELISA ). The levels of PDGF-B mRNA in peripheral blood mononuclear cells were measured by reverse transcription polymerase chain reaction (RT-PCR).Results The serum levels of PDGF-BB and the levels of PDGF-B mRNA in peripheral blood mononuclear cells were significantly higher in patients with lung cancer,which were(389.64±36.42)pg/ml and (0.928 7±0.094 2),respectively,vs. (28.36±8.35)pg/ml and(0.227 5±0.038 4)in normal controls (P<0.01).Conclusions PDGF-BB may play a role in the pathogenesis of lung cancer. Testing the levels of PDGF-BB、 PDGF-B mRNA may help with the diagnosis of lung cancer.更多还原