【摘要】 为了研究运动训练大鼠不同部位组织(重点是脂肪组织)和器官的自体荧光光谱特性。方法:通过激光诱导荧光光谱技术测量一次中等强度力竭运动后大鼠组织的自体荧光光谱,并在肠系膜部位的脂肪组织的三维荧光光谱中发现了和运动相关的特有荧光峰,并判别出此荧光峰对应的荧光物质是NADH(还原态烟酰胺腺嘌呤二核苷酸),且研究了运动状态与峰强的相关性。结果:中等强度运动后大鼠肠系膜部位的脂肪组织的能量代谢强于大网膜部位的脂肪组织、皮下脂肪组织,并且强于前腿肌肉、比目鱼肌及其它脏器;NADH自体荧光光谱特性是判断机体组织代谢程度的有效的指标之一。更多还原

【Abstract】 Aim: to investigate three-dimensional autofluorescence spectral characteristics of tissues and organs of different places of exercising rats.Methods: By laser-induced fluorescence(LIF) technique,three-dimensional autofluorescence spectral characteristics of tissues and organs of different places of exercising rats after treadmill exercise with 5° incline,32m/min,180 min/time are studied.Results: Specific exercise-related fluorescence peaks are discovered in the three-dimensional fluorescence spectra of adipose tissue and fluorescent substance corresponding to the fluorescence peaks is determined to be nicotine-amide adenine dinucleotide reduced(NADH).The study indicates that the energy metabolism of rats’ adipose tissue is stronger than other organs and the autofluorescence spectral characteristic is one of the effective indexes to judge the metabolic of muscle.更多还原