【摘要】 探讨检测血清细胞因子及肾综合征出血热(HFRS)病毒特异性抗体IgM和IgG的含量在HFRS发病机制及诊断中的意义。选择24例HFRS患者及30例健康人血清标本,采用生物素-亲和素-酶免疫技术检测IL-2、IL-6和TNF-,αELISA方法检测血清HFRS病毒特异性抗体IgM和IgG,并对其进行统计学分析。结果显示,ELISA法检测HFRS患者抗HFRS病毒IgM和IgG的阳性率分别为75.00%和50.00%,健康对照组的抗体阳性率为零;HFRS患者血清IL-2、IL-6、TNF-α的含量分别为10.88±2.31pg/mL、256.46±102.51pg/mL和45.63±5.32pg/mL,高于健康对照组0.59±0.24pg/mL(P<0.01)、53.8±19.21 pg/mL(P<0.01)和5.81±3.58 pg/mL(P<0.01)。结论:HFRS患者血清IL-2、IL-6和TNF-α及血清特异性抗体IgM和IgG的含量较健康人明显升高,检测这些指标对该病发病机理、诊断及预后评价有一定意义。更多还原

【Abstract】 To explore clinical significance of cytokine and specific antibodies of hemorrhagic fever with renal syndrome(HFRS) in the diagnosis of HFRS,the serum levels of IL-2,IL-6,and TNF-α in 24 HFRS patients and 30 healthy people were measured by the biotin-avidin-enzyme linked immunosorbent assay method.The specific anti-virus antibodies IgM and IgG were detected by ELISA.The results showed that the positive rate of anti-virus IgM and IgG in HFRS patients were 75.0% and 50.0 %,while in healthy control group was zero.The serum IL-2,IL-6 and TNF-α level in HFRS patients were dramatically higher than that of health control.The measurement of serum IL-2,IL-6 and TNF-α level and specific antibodies had an important role in the diagnosis and prognosis of hemorrhagic fever with renal syndrome.更多还原