【摘要】 在三孢布拉氏霉发酵48h添加3.6mmol/L2-(4-甲基苯氧)-三乙基胺(MPTA)可有效抑制96%β-胡萝卜素的合成;添加15g/L烟草可促进总类胡萝卜素的产量(170mg/L),但其对β-胡萝卜素的合成抑制作用低于MP-TA。L27(313)正交实验从抑制剂添加时间、添加量以及两种抑制剂的交互作用等方面考察了同时添加MPTA和烟草对番茄红素产量的影响。发酵48h同时添加烟草10g/L与MPTA2.2mmol/L,番茄红素的产量可达205mg/L(占总类胡萝卜素产量的94%)。更多还原

【Abstract】 The efficacy and optimization of the application of two inhibitors,tobacco and bleaching herbicide 2-(4-methylphenoxy)-triethylamine(MPTA),on lycopene productivity by Blakeslea trispora were investigated.The greatest yield of lycopene(170 mg/L)was observed when nicotine in the form of tobacco was added at 15 g/L at 48 hours old mated cultures,however,this addition did not inhibit(-carotene synthesis to the same extent as MPTA’s,but resulted in 53% increase in overall carotenoid synthesis.Lycopene biosynthesis was strongly stimulated(205 mg/L)at the expanse of(-carotene when 2.2 mmol/L MPTA and 10 g/L tobacco added together at 48 hours following inoculation.Because of the low quantity but great efficiency,this compound inhibitor of tobacco and MPTA will become a potential choice of inhibiting lycopene cyclase in Blakeslea trispora.更多还原