【摘要】 [目的]研究云芝糖肽(polysaccharopeptide,PSP)的镇痛作用并探讨其镇痛机制。[方法]60只SD大鼠,随机分为5组,即正常对照组、不同剂量PSP组和杜冷丁阳性对照组。PSP连续给药6d,用电刺激鼠尾-嘶叫法测定痛阈,观察PSP对痛阈的影响。同时测定PSP对下丘脑内侧基底部(MBH)白介素-2(IL-2)和白介素-2受体(IL-2R)表达的影响。[结果]大鼠经PSP治疗后,与正常组比,其痛阈升高,且有明显的量效关系。PSP能增加大鼠MBH区域IL-2免疫反应阳性细胞的数量,减少IL-2R阳性颗粒的分布。[结论]PSP有中枢性镇痛作用,其镇痛作用靶点可能位于下丘脑内侧基底部。机制可能与PSP刺激T细胞分泌IL-2,IL-2进入脑内并与MBH中的IL-2受体结合有关。更多还原

【Abstract】 [Objective]The study focus on the analgesic effect of polysaccharopeptide(PSP)and discuss the mechanism of this analgesia. [Methods]A total of 80 rats were divided randomly into high dose of psp,medium dose of psp,low doses of psp,positive control group,negative control group.After intragastric(ig) administration of different(high,medium and low)doses of PSP for 6 days,The tail stimulation-vocalization test was used to observe the analgesic effect of PSP.The IL-2 and IL-2R expression in mediobasal hypothalamus(MBH) was studied immunohistochemically.[Results]After intragastric(ig) administration of PSP for 6 days,the pain threshold was increased significantly and a dose-effect relationship was observed.After PSP administration the IL-2 expression in MBH was increased,while the IL-2R expression in MBH was decreased.[Conclusion]PSP has a definite analgesic effect and its analgesic site is in the MBH.The analgesia might be produced by the binding with the IL-2R in MBH by IL-2 secreted by T cells after PSP was injected into the brain.更多还原