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SPRR1A基因真核荧光表达载体的构建
Construction for fluorescent eukaryotic cell expression vector of small proline-rich repeat protein 1A gene
【摘要】 目的:构建可在真核细胞中表达SPRR1A(small proline-rich repeat protein1A)的荧光表达载体。方法:应用PCR技术扩增出编码SPRR1A的全长基因,将SPRR1A DNA克隆到pEGFP-N1质粒,通过抗性基因筛选阳性克隆,经酶切和测序鉴定。结果:酶切和DNA序列鉴定均证实插入片段与GenBank提供的序列(L05187)一致。结论:成功构建了SPRR1A真核荧光表达载体。
【Abstract】 Objective:To construct fluorescent eukaryotic expression vector of small proline-rich repeat protein 1A(SPRR1A)gene.Methods:The coding sequence of SPRR1A was amplified by polymerase chain reaction,the SPRR1A gene was cloned into plasmid pEGFP-N1,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results:Correct construction of pEGFP-N1-SPRR1A was identified by methods of restriction enzyme analysis and nucleotide sequence determination.Conclusions:Eukaryotic expression plasmid pEGFP-N1-SPRR1A has been successfully constructed.
【Key words】 neurofibrils; gene expression; regulation; small proline-rich repeat protein 1A; fluorescent eukaryotic cell expression vector;
- 【文献出处】 蚌埠医学院学报 ,Journal of Bengbu Medical College , 编辑部邮箱 ,2009年02期
- 【分类号】R651;R346
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