【摘要】 通过对胡萝卜SRAP反应体系中Mg2+浓度、dNTPs浓度、引物浓度以及模板DNA浓度的优化,结果表明,在20μL反应体系中,DNA模板最适浓度为3.00ng/mL,Mg2+最适浓度为2.25mmoL/L,引物最适浓度为0.30μmoL/L,dNTPs最适浓度为0.20mmoL/L。更多还原

【Abstract】 Through optimization of concentration of Mg2+,dNTPs,primers and template DNA,SRAP-PCR of carrot was established.The results showed that in the 20μL reaction mixture the optimum concentration of genomic DNA template,Mg2+,primers and dNTPs were 3.00ng/mL,2.25mmoL/L,0.30μmoL/L and 0.20mmoL/L.更多还原