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HPLC法测定参竹精胶囊中2,3,5,4,-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量

Determination of 2,3,5,4,-stilbene giucoside in Shengzhujing capsule by HPLC

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【作者】 程合理李文琪

【Author】 CHENG He-li,LI Wen-qi(The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022)

【机构】 安徽医科大学第一附属医院

【摘要】 目的建立参竹精胶囊中2,3,5,4,-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定方法。方法采用Sim pack CLC-C18柱(150 mm×4.6 mm),乙腈-水(20∶80),检测波长为320 nm。结果2,3,5,4,-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的回收率为98.39%,RSD=0.94%(n=5)。结论本法简便、快速、重现性好,可作为参竹精胶囊的定量分析方法。

【Abstract】 Aim To develop a method for determination of 2,3,5,4,-stilbene giucoside in Shengzhujing capsule.Method The analysis was performed on a Shim pack CLC-C18 column,with a mobile phase of acetonitrile-water(20∶80),the detection wavelength was 320 nm.Results Recovery of 2,3,5,4,-stilbene giucoside was 98.39%,RSD was 0.94%.Conclusion This method is simple,reproducible,and it can be used for the quantitative analysis of Shengzhujing capsule.

  • 【文献出处】 安徽医药 ,Anhui Medical and Pharmaceutical Journal , 编辑部邮箱 ,2009年06期
  • 【分类号】R286
  • 【被引频次】2
  • 【下载频次】33
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