【摘要】 目的通过研究基质金属蛋白酶MMP-2、MMP-9及其组织抑制剂TIMP-1、TIMP-2在不同类型滋养细胞疾病中的表达,探讨基质金属蛋白酶系统与滋养细胞及滋养细胞疾病的关系。方法应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(<12周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。结果(1)MMP-2在不同类型滋养细胞中的表达差异无统计学意义(χ2=0.926,P=0.921);(2)MMP-9在正常早孕绒毛组织、部分性葡萄胎、完全性葡萄胎、侵蚀性葡萄胎、绒毛膜癌中均100%表达,但随着滋养细胞恶性程度的增加其表达显著升高,差异有统计学意义(χ2=26.421,P=0.0001);(3)TIMP-1在正常早孕绒毛组织均有表达,而在部分性葡萄胎、完全性葡萄胎、侵蚀性葡萄胎、绒毛膜癌中分别有6.67%、5.45%、6.67%、12.50%不表达,且随着肿瘤恶性程度的升高,其染色程度和染色细胞百分率减少,差异有统计学意义(χ2=9.575,P=0.048);(4)TIMP-2在各组织中的表达差异无统计学意义(χ2=1.462,P=0.833)。结论随着滋养细胞疾病恶性程度的增加,MMP-9表达上调而TIMP-1表达下调。MMP-2、TIMP-2与滋养细胞浸润无密切关系。更多还原

【Abstract】 Objective To study the expression of matrix metallopreteinases(MMP-2,9) and tissue inhibitors of metallopreteinases(TIMP-1,2) in human gestational trophoblastic disease and their correlation with clinic pathological parameters in predicting the malignant transformation and metastasis.Methods Immunohistochemical streptavidin-peroxidase(S-P) method was used to detect the expression of MMP2,MMP-9,TIMP-1,TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles,55 cases of complete hydatidiform moles,15 cases of invasive moles and 8 cases of choriocarcinoma.Resealts(1) No significant difference with MMP-2 expression in all cases(χ2=0.926,P=0.921).(2) MMP-9 expression was detected in all types of trophoblastic cells,the staining was markedly increased with the development of the malignant transformation(χ2=26.421,P=0.0001).(3) Each cases of normal human cytotrophoblast cells expressed TIMP-1,but 6.67% of partial hydatidiform moles,5.45% of complete hydatidiform moles,6.67% of invasive moles and 12.50% of choriocarcinoma had no TIMP-1 expression,the staining was decreased with the development of the malignant transformation(χ2=9.575,P=0.048).(4) The expression of TIMP-2 in all cases has no significant difference(χ2=1.462,P=0.833).Conclusion Upregulation of the MMP-9 and downregulation of the TIMP-1 are related to the malignancy degree in the trophoblastic disease.MMP-2 and TIMP-2 might have no closely related to the invision of trophoblast.更多还原