【摘要】 [目的]筛选球根海棠叶培养中不同阶段的培养基配方。[方法]通过组织培养试验,从接种、继代增殖和生根培养3个阶段对球根海棠叶离体培养中的培养基配方进行筛选。[结果]在MS+6-BA1.00 mg/L+NAA0.20 mg/L+Ade 5.00 mg/L的培养基上接种诱导分化丛生芽,诱导成芽率达100%,在诱导分化培养基中添加6-BA有利于芽的形成,在配方中添加Ade能促使诱导;在MS+6-BA0.10mg/L+NAA0.01 mg/L的培养基上进行增殖,增殖系数高达40,且丛生芽整齐、健壮,最适用于生产;在1/2 MS+IAA0.80 mg/L培养基上生根壮苗,生根时间短、根系位置适宜、根系条数偏多,最为理想。[结论]筛选出了球根海棠叶离体培养时诱导阶段、增殖阶段和生根阶段的最佳培养基配方:在MS+6-BA1.00 mg/L+NAA0.20 mg/L+Ade 5.00 mg/L的培养基上接种诱导分化丛生芽,然后在MS+6-BA0.10 mg/L+NAA0.01 mg/L的培养基上快速增殖,在1/2 MS+IAA0.80 mg/L培养基上生根壮苗,再出瓶培养成生产用苗。更多还原

【Abstract】 [Objective] The selection of the optimum media for Begonia×tuberhybrida Voss leaf culture in vitro in different periods was experimented.[Method] The induction,subculture and rooting medium for its culture were experimented,respectively.[Results] In medium: MS + 6-BA 1.00 mg/L + NAA 0.20 mg/L + Ade 5.00 mg/L,the induction rate of bud Begonia×tuberhybrida leaf culture was 100%;6-BA added in the medium was beneficial to bud formation and Ade added in the medium promoted the induction.The multiplication coefficient of bud was as high as 40% in the subculture medium: MS+6-BA 0.10 mg/L+NAA 0.01 mg/L and the bud growth was order and strong,which was suitable for production practice.In rooting medium: 1/2 MS+ IAA 0.80 mg/L,the root regenerated was strong and multiple,and the time of rooting was short.[Conclusion] The procedure of Begonia×tuberhybrida Voss leaf culture in vitro was as follows: the bud was induced in the medium: MS + 6-BA 1.00 mg/L + NAA 0.20 mg/L + Ade 5.00 mg/L;propagated in the medium: MS + 6-BA 0.10 mg/L+NAA 0.01 mg/L and rooted in the medium: 1/2 MS + IAA 0.80 mg/L.更多还原