【摘要】 [目的]探究突变后植株叶片的生理生化变化。[方法]以1份条斑和颍花异常的水稻突变体为材料,研究该突变体生长阶段的几个不同时期几种酶活性变化和稻米的氨基酸含量变化。[结果]在条斑颖花突变体生长过程中,分蘖中期SOD的活性高于野生型,在开花前和开花末期的活性又低于野生型;突变体POD活性在3个时期中呈先上升后下降的趋势,在开花前期最高,野生型植株POD活性变化趋势与之相反;CAT在突变体的3个生长时期活性呈递减趋势,在分蘖中期活性很高,在野生型中活性变化趋势相反;MDA的活性在3个时期活性变化递增,野生型的活性相对较高;突变体中可溶性糖含量低;总氨基酸含量上升。[结论]突变体性状的表达与SOD、POD、CAT和MDA活性变化有一定关系,这些变化使突变体免于死亡,最终导致米质改变。更多还原

【Abstract】 [Objective]The experiment aimed to explore physiological and Biochemical changes of leaves after plants were mutated.[Method] A rice double mutants with stripes on stems,leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice.[Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase,but lower than that in wild plant before heading stage and heading telophase;the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity,however,the POD activity in wild plant showed the opposite change trend;the CAT activity presented degression at three stages,especially high at tillering metaphase,but reverse changes in wild plants;the MDA activity decreased at three stages but it was still higher than that in wild plant,besides,the soluble sugar content of mutant was lower but total amino acid content was increased.[Conclusion] The expression of mutant characteristics was correlated with SOD,POD,CAT and MDA activity changes and these changes made the mutant survive and rice quality change at last.更多还原