【摘要】 [目的]筛选出传统水提法提取当归多糖的最佳工艺及Savag’s法去蛋白时最佳操作次数。[方法]运用正交设计法设计提取工艺;采用Savag’s法去蛋白,并比较不同次数对多糖含量的影响。总糖含量测定采用改良苯酚-硫酸法;蛋白质含量测定采用考马斯亮蓝法;糖醛酸含量测定采用硫酸-咔唑法。[结果]提取的最佳工艺为:料水比1∶20、提取时间30 min、提取温度100℃;最佳去蛋白条件为:氯仿-正丁醇(5∶1),操作3次。[结论]该方法简便、易操作,适用于水溶性粗多糖的提取和检测。更多还原

【Abstract】 [Objective] The study aimed to select the best process of Chinese Angelica Polysaccharide(CAPS) with water extraction and the optimum operating times of removing protein with method of Savag’s.[Method] The orthogonal design was used to extract of CAPS.The Savag’s method was used to remove protein from polysaccharide and the polysaccharide contents were compared after the different removing times.The total polysaccharide contents were determined with method of phenol-sulfuric acid,the protein content determined by method of Coomassie brilliant blue,the uronic acid content with method of sulfate-carbazole.[Result] The optimum extracting process was that ratio of material to water was 1∶20,the extraction time was 30 minutes,extraction temperature was 100 ℃ and the optimum process of removing protein was 3 times(5∶1 ratio of chloroform:n-butanol).[Conclusion] The method was simple and easy to operate and was suitable for extracting and detecting of water-soluble polysaccharide.更多还原