【摘要】 [目的]探讨小麦不同取材部位对DNA提取质量和产率的影响。[方法]分别以小麦的种子、根尖和幼嫩叶片为材料,采用改良CTAB法分别提取小麦3个部位的基因组DNA,用0.8%的琼脂糖凝胶电泳检测DNA质量,用紫外分光光度计分别测定3个部位的DNA在A260和A280下的吸光值,根据A260/A280的值检测DNA的浓度和纯度,并以3个部位提取的DNA为模版进行RAPD扩增。[结果]采用小麦叶片提取DNA的产率最高,种子次之,根尖最低。3个部位提取的DNA纯度均相近,提取叶片获得的DNA浓度最大,为756μg/m l;其次是种子,为233μg/m l;根尖获得的DNA浓度最低,为90μg/m l。小麦不同部位提取的DNA模板对RAPD扩增没有明显影响,DNA浓度都能达到扩增的要求。[结论]小麦3个部位提取的DNA质量均较好,可以进行后续的酶切和PCR扩增等实验。更多还原

【Abstract】 [Objective] The aim was to discuss the effect of different sampling positions of wheat on the DNA quality and yield.[Method] With seeds,root tips and young leaves of wheat as tested materials,the genome DNA from 3 parts of wheat were extracted with modified CTAB method and examined with 0.8% agarose gel electrophoresis.The absorbance value of DNA from 3 parts was measured by ultraviolet spectrophotometer at A260 and A280 and the ratio of A260/A280 was used to determine the DNA purity and the mass concn.DNA isolated from 3 parts of wheat was used as the template for RAPD amplification.[Result] The yield of DNA extracted from wheat leaves was highest,that from the seeds was second,and that from the root tips was lowest.The DNA purity extracted from 3 parts of wheat was similar.The DNA concn.isolated from wheat leaves was the largest,being 756 μg/ml,followed by that from seeds,being 233 μg/ml that from the root was the lowest,being 90 μg/ml.The DNA template extracted from different parts of wheat had no significant effect on RAPD amplification and DNA concn.could achieve the amplification requirements.[Conclusion] The DNA qualities extracted from 3 parts of wheat were good and could be used in subsequent experiments such as digestion and PCR amplification.更多还原