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机油降解菌的分离及其降解特性研究

Study on the Isolation of Oil Degradation Bacteria and Its Degradation Characteristics

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【作者】 韩寒冰; 刘杰凤;

【Author】 HAN Han-bing et al (College of Chemistry and Life Science,Maoming University,Maoming,Guangdong 525000)

【机构】 茂名学院化学与生命科学学院;

【摘要】 [目的]分离主要的机油降解菌,并研究其降解特性。[方法]从茂名炼油厂附近长期被石油污染的土壤中,分离机油降解菌株,并对其进行形态特征和生理生化特性的分析。同时进一步研究了该菌株对机油降解特性及影响因素。[结果]分离得到3株机油降解菌,其中1株初步鉴定为芽孢杆菌属。该菌株在温度为40℃、pH值为8.0、机油浓度为100 mg/L、N源为NH4C l的条件下生长旺盛,降解率达到47.2%。[结论]利用生物降解的方法可以更有效地治理石油污染。

【Abstract】 [Objective] The research aimed to isolate the main oil degradation bacteria and study its degradation characteristics.[Method] Oil degradation strains were isolated from soil that was polluted by petroleum nearby Maoming Refinary.Their morphological,physiological and biochemical characteristics were analyzed.The degradation characteristics of these strains to machine oil and their influencing factors were studied.[Result] Three strains of degradation bacteria were isolated.And one strain was identified as Bacillas sp..The optimum culture conditions were as follows: pH value of 8.0,inoculation by NH4Cl as the N source in the culture medium and incubation at 40 ℃.When the proposed strain grew under these conditions,the degradation rate reached 47.2%.[Conclusion] Petroleum pollution could be effectively controlled by using biological degradation.

【基金】 茂名学院科学研究基金(203255)
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2009年21期
  • 【分类号】X172
  • 【被引频次】8
  • 【下载频次】133
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