【摘要】 [目的]建立健肝乐颗粒中芍药苷的含量测定方法。[方法]采用RP-HPLC法,色谱柱为ODS2-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(15∶85),流速为1.0 ml/min,检测波长为230 nm。[结果]芍药苷在0.16~0.83μg范围内与峰面积呈良好线性关系。加样回收率为99.20%,RSD为1.2%(n=6)。[结论]采用高效液相法对健肝乐颗粒中芍药苷进行含量测定,具有操作简单、准确、重复性好、精密度高、专属性强的特点,可用于健肝乐颗粒的质量检测。更多还原

【Abstract】 [Objective]The research aimed to establish a method for the determination of paeoniflor in Jianganle particle by RP-HPLC.[Method] The content of paeoniflor in Jianganle particle was determinated by using RP-HPLC.The separation was performed on a ODS2-C18 column(250 mm×4.6 mm,5 μm).The mobile phase was cetonitrile-water(15∶85)at the flow rate of 1.0 ml/min.The defection wavelength was 230 nm.[Result]The linear range of paeoniflorin was in the range of 0.16-0.83 μg;The addition recovery rate was 99.20%,RSD=1.2%(n=6).[Conclusion]The method for the determination of paeoniflor in Jianganle particle by RP-HPLC is simple,rapid,exclusive and accurate,and it is suitable for determination of paeoniflor of Jianganle particle.更多还原