【摘要】 [目的]检测猪CACNA2D1基因第14内含子的多态性,为猪肉质性状的分子标记辅助选择提供条件。[方法]采用PCR-SSCP检测猪CACNA2D1基因第14外显子和内含子在6个猪群中的DNA多态性,并分析不同基因型对猪眼肌和腿臀肌肉pH45的遗传效应。[结果]猪CACNA2D1基因第14内含子有3种基因型,即AA、AG和GG,在其序列(GenBank接受号:FJ156361)的1 145位碱基存在1个多态位点(G→A)。2χ独立性检验结果表明,这3种基因型在各猪群间的分布差异显著(P<0.05)。AA基因型金华×皮特兰F2资源家系猪与AG基因型猪在眼肌和腿臀肌肉pH45值上的差异显著(P<0.05)。[结论]猪CACNA2D1基因第14内含子的多态性影响猪眼肌和腿臀肌pH45值。更多还原

【Abstract】 [Objective] To detect polymorphism of the14th intron of porcine CACNA2D1 gene,and thus provide conditions for the marker-assisted selection of pork quality traits.[Method] The polymorphism of the14th exon and intron of porcine CACNA2D1 gene was detected in six breeds by PCR-SSCP.The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed.[Result] There were three kinds of genotypes,namely,AA,AG and GG,in the 14th intron of porcine CACNA2D1 gene.The polymorphic locus(G → A) was at the 1 145 nt of the sequence(GenBank Accepted No.FJ156361).The results of χ2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds(P<0.05).The pH45 values of eye muscle and ham joint muscle were significantly different between genotype AA and genotype AG of F2 generation from the Jianhua×Pietrain resource family(P<0.05).[Conclusion] The polymorphism of the4th intron of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.更多还原