【摘要】 [目的]对马尾藻中褐藻多酚进行研究。[方法]采用试验的方法,对广西北海涠洲岛马尾藻中多酚的提取、分离提纯、定性、定量分析以及抗菌的活性检验进行研究,考察不同的乙醇浓度、体积数及提取时间对褐藻多酚提取量的影响。[结果]40 ml 65%乙醇提取2 h对褐藻多酚的提取有显著影响,测得马尾藻中褐藻多酚的含量为7.777 mg/g;当纸片法的褐藻多酚浓度为140.055μg/张时,对沙门氏杆菌抑菌效果明显,大肠杆菌次之,但对枯草芽孢杆菌抑菌效果不明显,对金黄色葡萄球菌不敏感。[结论]乙醇的浓度、体积数和提取时间都会影响马尾藻多酚的提取。更多还原

【Abstract】 [Objective] The purpose was to study the polyphenols in Sargassum.[Method] The extraction,isolation and purification,examination,qualitative and quantitative analysis and antimicrobial activity test of polyphenols from the Sargassum in Weizhou Island of the North Sea in Guangxi Province were studied to investigate the effect of different concn.of alcohol,volumes of alcohol and extraction time on the extraction yield of polyphenols from Sargassum.[Result] The extractive method of extracting 2 h with 65% alcohol at 40 ml had obvious effect on the extraction of polyphenols from Sargassum,by which,the content of polyphenols from Sargassum was 7.777 mg/g.It was found that polypheols were significant against Salmonella and its effect against E.coli was the second,but it had no significant effect on Bacillus subtilis and had no effect on Staphylococcus aurells at all when the polypheols was 140.055 μg/sheet.[Conclusion] The concn.of alcohol,volumes of alcohol and extraction time all could effect the extraction of polyphenols from Sargassum.更多还原