【摘要】 [目的]筛选出适于工业化生产葛根素的提取、分离、纯化工艺路线。[方法]用熔点、红外光谱、核磁氢谱和紫外分光光度法分析鉴定提取的葛根素精品。[结果]葛根素精品的熔点为327~328℃,与葛根素标准品的熔点基本相同;在薄层色谱上,精品与标准品的斑点在紫外灯下均显蓝色荧光,Rf值均为0.31;精品的红外数据为IRUKMaBrx(cm-1):3 398、1 630、1 584、1 515、1 443、1 384、1 257、1 178、1 118、1 081、1 041,标准品的红外数据为IRUKMBaxr(cm-1):3 370、1 631、1 607、1 514、1 447、1 396、1 273、1 210、1 103、1 059、1 008;精品的核磁氢谱数据与文献报道相符;在250 nm处测定精品的吸光度,由回归方程计算出精品的葛根素含量为97%,液-液萃取法的葛根素精品的产率为1.41%。[结论]液-液萃取法分离纯化葛根素成本低,操作简单,可用于工业化生产。更多还原

【Abstract】 [Objective] The purpose of the study was to screen out the process route of extracting,isolating and purifying puerarin,which was suitable for industrial production.[Method] The extracted and refined puerarin was analyzed and identified by melting point method,infrared spectrophotometry,nuclear magnetic resonance hydrogen(NMR-H) spectroscopy and UV spectrophotometry.[Result] The melting point of refined puerarin was 327-328 ℃ and it was basically identical with the melting point of standard puerarin.On thin layer spectrum,both the dots of refined and standard puerarin showed blue fluorescence under UV-light lamp and both of their Rf values were 0.31.The infrared data of refined puerarin were IRUKBrMax(cm-1): 3 398,1 630,1 584,1 515,1 443,1 384,1 257,1 178,1 118,1 081 and 1 041 and that of standard puerarin was IRUKBrMax(cm-1): 3 370,1 631,1 607,1 514,1 447,1 396,1 273,1 210,1 103,1 059 and 1 008.The NMR-H data of refined puerarin accorded with literature reports.The absorbance of refined puerarin was detected at 250 nm,the puerarin content of the refined was calculated to be 97% through regression equation.The yield rate of refined puerarin of liquid-liqiud extraction was 1.41%.[Conclusion] Isolation and purification of puerarin by liquid-liqiud extraction was cheap in cost,simple to operate and could be used in industrial production.更多还原