【摘要】 [目的]为油菜潮霉素抗性基因的遗传转化提供依据。[方法]以油菜品种沪油19号、苏州青、五月慢的子叶和下胚轴为外植体,研究不同浓度潮霉素处理对其褐化率、出愈率的影响。[结果]潮霉素浓度为3 mg/L时,五月慢、沪油和苏州青的褐化率分别为87.6%、30.7%、40.1%;褐化的临界浓度均为6 mg/L。苏州青下胚轴对潮霉素的耐受性较强,其褐化的临界浓度高于沪油19号和五月慢。潮霉素浓度为6 mg/L时,沪油19号、苏州青、五月慢子叶的出愈率分别为3.7%、2.3%、0,下胚轴的出愈率分别为5.1%、8.1%、2.2%。[结论]以子叶为外植体时,3个品种适宜的潮霉素筛选浓度为6 mg/L;以下胚轴为外植体时,沪油19号和五月慢适宜的潮霉素筛选浓度为6 mg/L,苏州青为9 mg/L。更多还原

【Abstract】 [Objective] The aim was to provide the basis for genetic transformation of hygromycin resistance gene of Brassica napus L.[Method] With cotyledon and hypocotyl of B.napus varieties(Huyou 19,Suzhouqing,Wuyueman) as explants,the effects of treatments with different concn.of hygromycin on its browning rate and callus rate were studied.[Result] When hygromycin.was 3 mg/L,the browning rates of Wuyueman,Huyou 19 and Suzhouqing were 87.6%,30.7% and 40.1% resp.and the critical concentration.they browning was 6 mg/L.The tolerance of hypocotyl of Suzhouqing to hygromycin was stronger and the critical concentration of its browning was higher than that of Huyou 19 and Wuyueman.When hygromycin.was 6 mg/L,the callus rates of cotyledon of Huyou 19,Suzhouqing,Wuyueman were 3.7%,2.3% and 0 resp.and those of hypocotyl were 5.1%,8.1% and 2.2% resp.[Conclusion] When cotyledon of B.napus was taken as explants,the optimum screening concn.of 3 varieties on hygromycin was 6 mg/L,when its hypocotyl was taken as explants,the optimum screening concn.of Huyou 19 and Wuyueman on hygromycin was 6 mg/L and that of Suzhouqing was 9 mg/L.更多还原