【摘要】 背景与目的:探讨巯基乙酸(thioglycolic acid,TGA)对昆明小鼠排卵以及卵母细胞体内成熟的影响。材料与方法:选取昆明小鼠12只,按体重随机分成4组,每组3只,分别为对照组和TGA高(151.25 mg/kg)、中(75.625 mg/kg)、低(37.812 5 mg/kg)3个剂量组。给小鼠皮肤染毒TGA的同时腹腔注射10 IU人绒毛膜促性腺激素(hCG),14 h后,处死小鼠收集卵母细胞进行小鼠排卵计数,采用免疫荧光染色法观察成熟卵母细胞内纺锤体与皮质颗粒(cortical germinal,CG)的分布。结果:TGA高、中剂量组排卵数明显低于对照组及低剂量组(P<0.01及P<0.05);对照组和低剂量组小鼠卵母细胞纺锤体呈双极状,中、高剂量组纺锤体逐渐呈桶状,形态发生了明显改变,且随着TGA剂量的增加,纺锤体的面积有增加趋势;TGA对CG的膜下分布以及无皮质颗粒区(cortical germinal freedomain,CGFD)的形成在形态上没有明显影响。结论:经皮肤给TGA能抑制小鼠排卵,并对卵母细胞成熟有一定影响。更多还原

【Abstract】 BACKGROUND AND AIM: To explore the effect of thioglycolic acid(TGA) on mouse ovulation and in vivo maturation of oocytes.MATERIALS AND METHODS: Kunming mice were used in this study.In each experiment twelve mice were allocated to four groups,control,37.812 5,75.62 5 and 151.25 mg/kg TGA groups.The mice received various doses of TGA percutaneously,and intraperitonea 10 IU human chorionic gonadotrophin simultaneously 14 hours later the number of ovulated oocytes was counted.Immunofluenscence staining was used to label spindle and cortical granule(CG) in the matured oocytes.RESULTS: Compared to the control,only 151.25 mg/kg TGA group showed significantly decreased ovulated oocytes(P<0.01),and also significantly lower than that in 37.812 5 mg/kg group(P<0.05).The spindle in control and 37.812 5 mg/kg groups were bipolar,but had a barrel configuration in 75.625 mg/ml and 151.25 mg/ml groups.The area of spindle increased with increasing TGA dose.TGA had no significant influence on the distribution of CGs and the formation of cortical granule-free domain(CGFD).CONCLUSION: Percutaneous administration of TGA inhibited ovulation of mouse and inhibited oocyte maturation in vivo.更多还原