【摘要】 目的:探讨非选择性环氧合酶-2(cyclooxygenese-2,COX-2)抑制剂阿司匹林赖氨酸盐对乳腺癌MDA-MB-231细胞株COX-2、基质金属蛋白酶-9(matrix metalloproteinaseses-9,MMP-9)蛋白表达的影响。方法:不同浓度的阿司匹林赖氨酸盐作用于MDA-MB-231细胞24h后,HE染色观察细胞形态学的变化;采用MTT法检测阿司匹林赖氨酸盐对MDA-MB-231乳腺癌细胞增殖的抑制作用;应用Western印迹法分别检测阿司匹林赖氨酸盐对人乳腺癌MDA-MB-231细胞株COX-2、MMP-9蛋白表达的影响。结果:光学显微镜下阿司匹林赖氨酸盐处理组的细胞密度减小,细胞变圆,胞核染色变浅。阿司匹林赖氨酸盐对MDA-MB-231乳腺癌细胞的增殖有明显抑制作用(P<0.01)。与对照组相比,阿司匹林赖氨酸盐可显著抑制MDA-MB-231乳腺癌细胞COX-2、MMP-9蛋白的表达,并呈剂量-依赖效应(P<0.05)。结论:阿司匹林赖氨酸盐可抑制MDA-MB-231乳腺癌细胞COX-2、MMP-9蛋白的表达。更多还原

【Abstract】 Objective:To investigate the effects of aspisol on expressions of cyclooxygenese-2(COX-2),matrix metalloproteina-seses-9(MMP-9) proteins in human breast cancer MDA-MB-231 cells.Methods:MDA-MB-231 cells were incubated with aspisol at various concentrations for 24 h.The morphological changes of MDA-MB-231 cells were evaluated by HE staining.The inhibitory effects of aspisol on the proliferation of MDA-MB-231 cells were determined by MTT assay.The effects of aspisol on the expressions of COX-2 and MMP-9 in MDA-MB-231 cells were measured by Western blotting.Results:Under light microscope the cells in aspisol treatment groups showed decreased density,round shape,and light staining of nucleus.Aspisol significantly inhibited the proliferation of MDA-MB-231 cells(P<0.01).Compared with the control group,aspisol dose-dependently inhibited the expressions of COX-2 and MMP-9 protein in MDA-MB-231 cells(P<0.05).Conclusion:This study suggested that aspisol inhibited the expressions of COX-2 and MMP-9 protein in breast cancer MDA-MB-231 cells.更多还原