【摘要】 以黑松花粉粒为研究材料,经过离子注入后对其细胞内的超氧阴离子自由基(O2-.)、丙二醛(MDA)含量、细胞膜透性和抗氧化酶(SOD、POD和CAT)活性的动态变化进行了检测.研究结果表明,氮离子注入对花粉粒内O2-.的产生速率、MDA含量、SOD、POD和CAT的活性均有明显的影响.随着离子注入剂量的增加,在花粉粒内O2-.的产生速率会持续加快;MDA的含量会相应增多;花粉粒细胞膜的伤害度表现出持续加重;SOD的活性呈现出先增加后降低的变化趋势;POD和CAT活性会持续降低.更多还原

【Abstract】 The accumulation of superoxide free anion(O2·-) and malondialdehyde(MDA),the relative membrane permeability,as well as activities of some protective enzymes such as superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) are measured in Pinus thunbergi pollen following N+ implantation,with an aim to investigate the physiological mechanism of cell damage caused by ion implantation.The results show that the physiological indexes are obviously effected by N+ implantation.Following increment of N+ implantation dose,the productive speed of the superoxide free anion in the pollen expresses faster;malondialdehyde much more;the damaged degree of the membrane more evident.Otherwise,the numerical measure of SOD in the pollen show the tendency of increase in initial stage and decrease in following stage;the quantity of POD and CAT display continuous reduction,accompanying to raise N+ implantation dose.更多还原