【摘要】 目的研究体外扩增后人脂肪源干细胞(Adipose-derived stem cells,ADSCs)在体外的免疫调节功能,以期以少量组织获得足够量的低免疫原性且具有免疫调节功能的脂肪源干细胞,用于组织修复及细胞治疗。方法流式细胞仪检测扩增至第2代及第5代的人脂肪源干细胞表面标记物,观察在扩增的过程中ADSCs表面标记物的变化;同时,检测扩增至第5代的ADSCs在体外抑制淋巴细胞增殖的能力,以评价ADSCs的免疫原性及免疫调节功能。结果ADSCs在扩增过程造血干细胞标记物表达降低,间质细胞表型及内皮细胞表型变化无显著性差异,同时第5代的ADSCs在体外不会引起淋巴细胞的增殖,并且在混合淋巴细胞反应中可以抑制淋巴细胞的增殖。结论扩增中的ADSCs逐渐表达均一的间充质干细胞的表面标记物,并在扩增至第5代时仍保持有低免疫原性及免疫调节的能力。更多还原

【Abstract】 Objective To study the immunological properties of human adipose-derived stem cells (ADSCs) with passage in vitro, in order to have enough stem cells with low immunogenicity and immune regulation function for the use in the organization repair and cell therapy. Methods Flow cytometry was performed to show the changes with the passage of ADSCs, and its ability to inhibit proliferation of lymphocyte with the fifth passage of ADSCs in vitro. Results The expression of the stromal-associated markers and endothelial-associated markers on human adipose-derived cells did not change significantly with passage, but the hematopoietic stem cell-associated markers decreased, ADSCs still has function to inhibit the mixed lymphocyte cultures after expansion. Conclusion ADSCs still maintain the the stromal-associated markers and the immunological properties after expansion.更多还原