【摘要】 目的研究狭叶茶(Camellia angustifolia Chang)提取物对小鼠糖耐受量的影响及其作用机制。方法分别采用葡萄糖、蔗糖及淀粉负荷方法制备小鼠糖耐量模型,检测给药后不同时间的血糖浓度,观察狭叶茶提取物对血糖水平的影响。体外实验测定狭叶茶提取物对小鼠小肠黏膜糖苷酶的抑制作用。建立狭叶茶提取物对不同α-葡萄糖苷酶活性抑制实验并观察其作用。结果与正常对照组相比,狭叶茶提取物可以缓解餐后血糖升高的趋势,并在糖苷酶活性实验中表现出不同程度的抑制作用。结论狭叶茶提取物可有效地降低餐后血糖水平,其作用机制可能部分来自于抑制α-葡萄糖苷酶活性。更多还原

【Abstract】 Objective To investigate the hypoglycaemic activity of extracts of Camellia angustifolia Chang(CA.ex.)in mice and to study its mechanism.Methods Oral glucose tolerance test was used to examine the hypoglycaemic effects of CA.ex,the small intestinal mucosa of mice was scraped to make the homogenate for the preparation ofα-glucosidases, the amount of glucose was measured to indicate the activities ofα-glucosidases.Results Compared with those in control group,the blood glucose levels were significantly decreased in the CA.ex.treatment group after giving sucrose and starch,in vitro,which showed that CA.ex,inhibited the activity ofα-glucosidases.Conclusion Oral use of CA.ex. leads to a decrease of blood glucose level and its mechanism may be concerned with the inhibition of the activities ofα- glucosidases.更多还原