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Liquid-nitrogen cryopreservation of three kinds of autotrophicbioleaching bacteria

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【Author】 WU Xue-ling~1 XIN Xiao-hong~2 JIANG Ying LIANG Ren-xing~1 YUAN Peng~1 FANG Cheng-xiang 1.Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China;2.China Center for Type Culture Collection,College of Life Science,Wuhan University,Wuhan 430072,China

【机构】 Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University； China Center for Type Culture Collection,College of Life Science,Wuhan University；

【摘要】 <正>Three kinds of autotrophic bioleaching bacteria strains,including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans(A.ferrooxidans),mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans(A.thiooxidans),and moderately thermophilic sulfur-oxidizing bacteria.4cidianus brierleyi,were cryopreserved in liquid nitrogen and their ferrous ion-or sulfur-oxidizing activities were investigated and compared with the original ones.The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation.Glycerin was used as cryoprotective agent.In conclusion,liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.更多还原

【Abstract】 Three kinds of autotrophic bioleaching bacteria strains,including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans(A.ferrooxidans),mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans(A.thiooxidans),and moderately thermophilic sulfur-oxidizing bacteria.4cidianus brierleyi,were cryopreserved in liquid nitrogen and their ferrous ion-or sulfur-oxidizing activities were investigated and compared with the original ones.The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation.Glycerin was used as cryoprotective agent.In conclusion,liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.更多还原

【关键词】 A.ferrooxidans； A.thiooxidans； Acidianus brierleyi； cryopreservation； liquid nitrogen； growth activities；
【Key words】 A.ferrooxidans； A.thiooxidans； Acidianus brierleyi； cryopreservation； liquid nitrogen； growth activities；

【基金】 Project(50621063)supported by Chinese Science Foundation for Distinguished Group;Project(2004CB619201)supported by the National Basic Research Program of China

【文献出处】 Transactions of Nonferrous Metals Society of China ,中国有色金属学会会刊(英文版) , 编辑部邮箱 ,2008年06期

【分类号】TF18
【被引频次】5
【下载频次】25

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