【摘要】 目的:建立心脑贯通滴丸（三七、银杏叶）中三七皂苷R₁、人参皂苷Rg₁、Rb₁的含量测定HPLC-ELSD方法。方法:色谱柱Agilent ODS C₁₈柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水,梯度洗脱,ELSD漂移管温度70℃;氮气流速2.0 L·min^-1;柱温35℃。结果:3种皂苷,线性范围分别为:三七皂苷R₁0.26～2.6μg（r=0.999 2）,人参皂苷Rg₁ 0.8～8.0μg（r= 0.999 8）,人参皂苷Rb₁ 0.7～7.4μg（r=0.999 0）,平均回收率分别为100.6%,102.5%和103.3%;RSD分别为3.3%,3.8%和3.9%（n=5）。结论:结果准确,便于操作,可作为心脑贯通滴丸的质量控制方法之一。更多还原

【Abstract】 Objective:To establish a method for determination the contents of notoginsenoside R₁,ginsenoside Rg₁ and Rh₁ in Xinnao Guantong dripping pills by HPLC-ELSD.Method:Agilent ODS C₁₈ column （250 mm×4.6 mm,5μm）was used,the acetoni- trile-water as mobile phase,gradient elution,the drift tube temperature was 70℃.The nitrogen as carrier gas,the flow rate was 2.0 L·min^-1 ELSD as detector.Result:The linear ranges of notoginsenoside R₁,ginsenoside Rg₁ and ginsenoside Rb₁ were at 0.26-2.6μg（r=0.999 2）,0.8-8.0μg（r=0.999 8） and 0.7-7.4μg（r=0.999 0）,the average recoveries（n=5） were 100.6%, 102.5%0 and 103.3% with RSD 3.3%,3.8% and 3.9%,respectively.Conclusion:The method is simple and rapid,it can be used for quality control of Xinnao Guantong dripping pills.更多还原