【摘要】 目的:建立羌活解表胶囊中盐酸小檗碱的含量测定的HPLC法。方法:用Diamonsil C₁₈色谱柱（200 mm×4.6 mm,5μm）;乙腈-0.05 mol·L^-1磷酸二氢钾溶液（26:74）为流动相;流速1.0 ml·min^-1,检测波长345 nm,柱温30℃。结果:盐酸小檗碱在0.08～0.88μg范围内呈良好的线性关系,r=0.999 9。平均回收率为102.3%,RSD为2.4%（n=6）。结论:方法简单、可靠、专属性强,可以作为控制羌活解表胶囊质量的方法。更多还原

【Abstract】 Objective:To establish a HPLC method for the Determination of Berberine Hydrochloride in Qianghuojiebiao Capsule. Method:Diamonsil C₁₈column（200mm×4.6mm,5μm）was used,the acetonitrile-0.05 mol·L^-1potassium dihydrogen phosphate solution（26:74）as mobile phase.The flow rate was 1.0 ml·min^-1,The detaction wavelength was 345 nm.The column temperature was 30℃.Result:The linear range of Berberine hydrochloride was 0.08-0.88μg,r=0.9999.The average recovery was 102.3%, RSD 2.4%（n=6）.Conclusion:The method is reliable,accurate and specific.It can be used for quality control of Qianghuojiebiao Capsule.更多还原