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HPLC法测定活血胶囊中丹参酮Ⅱ_A的含量

Determination of Tanshinone Ⅱ_A in Huoxue Capsule by HPLC

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【作者】 黄华轼; 吴素珍;

【Author】 Huang Huashi Wu Suzhen (Ba’oan People’s Hospital,Guangdong Shenzhen 518101,China)

【机构】 深圳市宝安人民医院;

【摘要】 目的:建立活血胶囊中的丹参酮ⅡA的含量测定方法。方法:色谱柱:Kromasil C18(250 mm×4.6 mm,5μm);流动相为甲醇-水(75:25);检测波长为270 nm;流速:1.0ml·min-1;柱温:35℃.结果:丹参酮ⅡA在72.0~360.0ng范围内呈线性关系,r=0.999 9,平均回收率为98.18%,RSD=1.92%(n=6)。结论:该方法简便、准确、可靠,可用于活血胶囊中丹参酮ⅡA的含量测定。

【Abstract】 Objective:To determine the content of TanshinoneⅡA in Huoxue Capsule.Method:The kromasil C18 column (250 mm×4.6 mm,5μm) was used,the mobile phase consisted of methanol-water(75:25),the column temperature was 35℃;the flow rate was 1.0 ml·min-1 and the detection wavelength was at 270 nm.Result:TanshinoneⅡA showed a good linearity within the range of 72.0~360.0 ng.The average recovery was 98.18% and RSD was 1.92% (n=6).Conclusion:The method was rapid,accurate and can be used for quality control of TanshinoneⅡA in Huoxue Capsule.

【关键词】 活血胶囊; 高效液相色谱法; 丹参酮Ⅱ_A;
【Key words】 Huoxue Capsule; HPLC; TanshinoneⅡ_A;
  • 【分类号】R286.0
  • 【被引频次】1
  • 【下载频次】34
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