【摘要】 目的:观察滋肾解毒中药对Ⅳ型狼疮性肾炎(LN)血清干预下肾小球系膜细胞(GMC)中Ⅳ型胶原(ColⅣ)mRNA表达的影响,从细胞分子生物学水平探讨滋肾解毒中药治疗LN的机制。方法:结合血清药理学方法,分空白对照组、对照组血清+15%LN血清组、低、中、高浓度含药血清+15%LN血清组5组观察,RT-PCR检测GMC中ColⅣ mRNA的表达。结果:含药血清可明显抑制GMC中ColⅣ mRNA的表达,以高浓度组最显著。结论:滋肾解毒中药的含药血清在15%Ⅳ型LN患者血清诱导下可明显抑制ColⅣ mRNA的表达,从而抑制GMC增殖和细胞外基质积聚,这可能是滋肾解毒中药治疗LN的分子机理。更多还原

【Abstract】 Objective:To observe the effects of Chinese crude drug of Invigorating the Kidney,Heat-clearing and Detoxicating(IKHD)on collagen Ⅳ(Col Ⅳ)mRNA expression of glomerular mesangial cell(GMC)induced by type-Ⅳ Lupus nephritis(LN).To explore the mechanism of the method of IKHD on the level of cell and molecular biology.Methods:With the method of blood serum pharmacology,5 groups were observed:blank,control serum + 15% serum of type-Ⅳ LN patients,low,medium and high concentration serum which contained Chinese medicine of IKHD + 15% serum of type-Ⅳ LN patients.The method of RT-PCR was used to detect expression of Col ⅣmRNA.Results:Different concentration serum containing traditional Chinese medicine of IKHD could inhibit the expression of Col ⅣmRNA.The effect of high concentration serum was the most notable.Conclusion:Being intervened by the 15% serum of type-Ⅳ LN patients,the serum containing traditional Chinese medicine of IKHD can inhibit the expression of Col ⅣmRNA.Through this,it may inhibit the proliferation of GMC and the accumulation of extracellular matrix(ECM).This may be the molecular mechanism of TCM of IKHD curing LN.更多还原