【摘要】 目的:探讨非光敏中药组方中药2号临床治疗白癜风有效的可能机制。方法:中药2号乙醇提取液外搽大鼠背部皮肤,并以75%乙醇外搽作为阴性对照,4周后取组织活检。采用免疫组化检测大鼠表皮中S100、干细胞因子受体(c-K IT)和蛋白酶激活受体-2(PAR-2)表达量的变化。结果:与75%乙醇外搽相比,中药2号外搽后S100阳性细胞数无显著性差异(P>0.05);c-K IT和PAR-2阳性细胞数均有增加,差异有显著性(P<0.05)。讨论:中药2号外搽治疗白癜风有效的机理可能与促进细胞移行、黑素转运有关;而与黑素细胞增殖无关。更多还原

【Abstract】 Objective:To explore the possible mechanism of extracts of non-photosensitive traditional Chinese Medicinal Herbs(No.2 Traditional Chinese Medicinal Herbs,No.2 TCMHs)in the treatmentof vitiligo.Methods:No.2TCMHs were extracted by alcohol,then the rat skin was treated by the ethanol extracts twice a day,and ethanol was used as negative control.Four weeks later,the skin specimens were obtained.Immunohistochemistry method was used to detect the S100,Kit-tyrosine kinase receptor(c-KIT)and proteinase-activated receptor-2(PAR-2)protein expression in these specimens.Results:Compared with the ethanol group,there was a significant increase in the positive rates of c-KIT and PAR-2 expression in No.2TCMHs treated group(P<0.05),while there was no significant increase of the S100 positive cells(P>0.05).Conclusion:Our results suggest that the efficacy of No.2TCMHs in the treatment of vitiligo may be related to its ability to enhance cell migration and melanin transfer,and not related to the increasing of melanocytes.更多还原