【摘要】 目的:从蛋白质组学角度研究中医肝风内动证的可能的标志性蛋白———硫氧环蛋白过氧化物酶(Peroxiredoxin/Thioredoxin peroxidase,TPx)在中医肝风内动三亚型中的表达,探讨其与中医肝风内动证的关系。方法:设肝阳化风证、血虚生风证、阴虚生风证、健康人组4组,抽取外周静脉血淋巴细胞并提取总蛋白行二维凝胶电泳,PDquestV7.3.1软件分析,质谱分析鉴定,找出各组共有的差异蛋白。结果:鉴定硫氧环蛋白过氧化物酶(Peroxiredoxin/Thioredoxin peroxidase,TPx)为中医肝风内动证与健康人组比较共同的差异蛋白。结论:硫氧环蛋白过氧化物酶(TPx)可能是中医肝风内动证的特异的标志蛋白。更多还原

【Abstract】 Objective:use Proteomic method to discover probable mark protein of Endogenous Liver Wind Syndrome—Peroxiredoxin/Thioredoxin peroxidase(TPx),study its express in three subtypes of Endogenous Liver Wind Syndrome,and approach their internal relation.Methods:Set up 4 groups,Gan Yang Hua Feng Syndrome,Xue Xu Sheng Feng Syndrome,Yin Xu Sheng Feng Syndrome and healthy humans,Extract total protein from lymphocyte of peripheral venous blood,two dimensional electrophoresis was used to separate total protein,software PDquestV7.3.1 analyzed,and identified by peptide mass fingerprint(PMF) based on Matrix assisted laser desorption/ionizationtime of flightmassspec trometry(MALDI-TOF-MS) and database.Results:TPx was identified to be the common different protein.Conclusion:TPx is probable the specific mark protein of Endogenous Liver Wind Syndrome.更多还原