【摘要】 目的:尝试以电视辅助胸腔镜外科技术(VATS)为新方法,探索建立慢性微创心肌梗死模型的制作方法。方法:在电视辅助胸腔镜下找到家犬的冠状动脉前降支后,以钛夹钳夹,造成心肌梗死并进行长期观察,系统监测了模型组与对照组的肢体导联ST段抬高值、全血白细胞计数(WBC)、血清OMP、肌酸激酶(CK)、心肌肌钙蛋白I(cTnI)含量、梗塞区面积占心室面积的百分比(%)等一系列生理、生化指标,同时观察了两组的Masson三色染色病理学表现。结果:模型家犬的ST段抬高值、WBC、血清OMP、CK、cTnI含量、梗塞区面积占心室面积的百分比(%)与对照组比较,均有显著性差异。Masson三色染色与cTnI免疫组化染色也为梗死区的形成提供了直接的证据。结论:VATS下钛夹夹闭犬冠脉前降支能够建立家犬的慢性微创心肌梗死模型。更多还原

【Abstract】 Objective: The present study tried to make a cardiovascular pharmacodynamic platform by new method-video-assisted thoracic surgery (VATS). Methods: pericardium was cut and 2～3 titanium nips would be used and a serials of examinations were made. Results: a serials of examinations had clear change. Conclusion: All the results showed that a new canine model of acute could be established by titanium nips clamped onto selected coronary arteries under video-assisted thoracoscopy.更多还原