【摘要】 目的:研究大孔吸附树脂分离纯化玫瑰花中原花青素的最优工艺条件。方法:以静态饱和吸附量和解析率为指标,对五种大孔吸附树脂(D101、D1300、NKA、AB-8、NKA-Ⅱ)进行筛选,并以原花青素纯度为指标,采用正交试验法,分别考察了上样浓度、pH、洗脱液浓度和洗脱流速对纯化效果的影响。结果:D101型树脂综合性能最佳,适合纯化玫瑰花中原花青素,最优工艺条件为:上样浓度为1.25 mg/ml,上样pH为2,洗脱液为70%乙醇水溶液,洗脱流速为2 ml/min。结论:本研究可为分离纯化玫瑰花中原花青素提供方法。更多还原

【Abstract】 Objective: To study the separation and purification technology for proanthocyanidins from rose and establish the best operating conditions.Methods: Evaluated by static adsorption capacity and elution ratio,five types of macroporous resins including D101,D1300,NKA,AB-8,NKA-Ⅱ were tested to separate and purify proanthocyanidins.And evaluated by product purity,the concentration of extract sample,pH of extract sample,concentration of eluant and flow rate of elution had been investigated.Results: D101 type macroporous resin showed the best property and was suitable for purifying proanthocyanidins from rose.The best operating conditions were as follows: 1.25 mg/ml as the concentration of extract sample,2 as the pH value of extract sample,70% ethanol equeous solution as the eluant and 2 ml/min as the flow rate of elution.Conclusion: This study can supply a method to separate and purify proanthocyanidins from rose.更多还原