【摘要】 目的:筛选陆英具有保肝降酶作用的优选提取工艺,并探讨陆英中保肝降酶的活性部位。方法:采用了小鼠急性CCl4肝损伤模型,对陆英不同提取工艺所得的提取物进行保肝降酶药效学筛选,并把筛选出的最佳工艺提取物用不同溶剂萃取法和大孔吸附树脂分离法对活性部位进行了探索。结果:陆英75%乙醇提取物能非常显著地对抗CCl4导致小鼠肝损伤,效果优于其他提取工艺;陆英75%乙醇提取物经乙酸乙酯萃取部分能减少CCl4导致小鼠肝损伤,效果明显弱于陆英75%乙醇总提物,而石油醚、正丁醇和水萃取部分则无明显效果;陆英75%乙醇提取物经大孔吸附树脂纯化后30%、60%和95%乙醇洗脱液的高剂量和低剂量组均能对抗CCl4导致小鼠肝损伤,但30%乙醇洗脱液的作用最显著,并呈现剂量依赖性,且效果与陆英75%乙醇总提物相似。结论:75%乙醇为陆英的优选提取溶剂,陆英75%乙醇提取物若经不同极性溶剂萃取,活性部位为乙酸乙酯萃取部分;若经大孔树脂纯化,则活性有效部分主要集中在30%乙醇洗脱液部分。更多还原

【Abstract】 Objective: To investigate the best extraction process of Sambucus chinensis against hepatitis and research on its active part.Methods: We studied the protective effects of different extracts of Sambucus chinensis distilled by different extraction process on mice acute hepatic injury induced by CCl4,and searched for the active part of Sambucus chinensis against hepatitis from the best extract by extraction with different solvent and purification with macroporous adsorption resin,then studied their protective effects on mice acute hepatic injury induced by CCl4.Results:The extraction of Sambucus chinensis by 75% alcohol showed very significantly protective effects on mice acute hepatic injury induced by CCl4 and the effects were better than that of other extraction process.The extraction eluted by 30% alcohol after purification with macroporous adsorption resin and extracted by EtOAc in the extraction of Sambucus chinensis by 75% alcohol all showed significantly protective effects on mice acute hepatic injury induced by CCl4,and the effects of the extraction eluted by 30% alcohol after purification with macroporous adsoption resin were better than extraction with EtOAc.Conclusions:The best extraction solvent is 75% alcohol.The active part of Sambucus chinensis against hepatitis is the extraction eluted by 30% alcohol after purification with macroporous adsorption resin and extraction with EtOAc.更多还原