【摘要】 应用RP-HPLC技术分析毛蚶中的抗癌活性肽。毛蚶活性肽的最优分离条件为:温度:30℃;波长:280nm;流速:1.0 ml/min;流动相A为80%乙腈(含0.1%TFA),流动相B为0.1%TFA水溶液,梯度洗脱。应用上述条件,成功地得到了10批样品的指纹图谱,并确定了14个共有峰。更多还原

【Abstract】 RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30℃;wavelength: 280 nm;flow rate: 1.0 ml/min;Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid(TFA) and solvent B contained 0.1% TFA.In this condition,ten samples′ fingerprints were gained,in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.更多还原