【摘要】 目的:探讨采用微透析取样技术实现皮肤局部青藤碱浓度动态测定的可行性和优越性。方法:以大鼠为研究对象,分别进行经皮及腹腔注射给药,采用微透析技术取样,高效液相色谱法分析,测定皮肤局部青藤碱浓度的动态变化。结果:在现有检测条件下,经皮给药后,皮肤局部药物浓度难以检测,而腹腔注射给药后,皮肤局部药物浓度的变化呈现相应的变化趋势。结论:微透析取样技术可以实现皮肤局部药物浓度的动态测定,与传统的皮肤药物浓度测定方法相比,具有明显的优势。更多还原

【Abstract】 Objective:To explore the feasibility and advantages of using microdialysis as sampling method for dynamic determination of sinomenine in topical skin.Methods:In this study,sinomenine was administered to the rats by transdermal drug delivery system and celiac injection.With microdialysis technique for sampling,the concentration of sinomenine in dialysate was determined by high performance liquid chromatography(HPLC).Results:Under existing determination condition,topical drug concentration in the skin of rats was hard to be detected after sinomenine administered to the skin,but it could be detected after celiac injection.Conclusion:Microdialysis sampling method can be used to determine topical drug concentration in the skin dynamically,and this method is better than traditional methods obviously.更多还原