【摘要】 目的:研究防风多糖的抗氧化活性。方法:采用水提醇沉法提取防风多糖,利用CTAB沉淀法对防风多糖进行分离,得到酸性防风多糖(A-SPS)和中性防风多糖(N-SPS)。在体外化学模拟条件下,研究不同防风多糖的总还原能力以及对超氧阴离子(O2-.)、羟基自由基(.OH)、1,1-二苯基-2苦苯肼自由基(DPPH.)的清除作用和对Fe2-诱导的脂质过氧化反应的抑制作用。结果:防风多糖具有一定的清除自由基、抑制脂质过氧化的能力,其中对.OH和DPPH.具有较强的清除作用。在几种防风多糖中,以A-SPS的效果较好,当实验浓度为8 mg/m l时,对.OH和DPPH.的清除率均接近于70%。结论:A-SPS具有较强的抗氧化作用,可能是防风多糖的主要活性组分,可作为潜在天然抗氧化剂应用于食品和医药工业中。更多还原

【Abstract】 Objective:To research the antioxidant activity of polysaccharides from Saposhnikovia divaricata(Turez.)Schischk.Method:Polysaccharides from Saposhnikovia divaricata(Turez.)Schischk were obtained through hot water extracting and ethanol precipitating method.Using CTAB as precipitant,Saposhnikovia polysaccharide was separated into acid Saposhnikovia polysaccharide(A-SPS)and neutral Saposhnikovia polysaccharide(N-SPS).The total reducing power,scavenging effect to superoixide anion(O-2·),hydroxyl radical(·OH),1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and inhibiting effect to lipid-peroxidation induced by Fe2+ of different Saposhnikovia polysaccharides were measured in vitro chemical simulated systems for evaluating their antioxidant activity.Results:Saposhnikovia polysaccharides had some functions of scavenging free radicals and inhibiting lipid peroxidation,and scavenging effects on DPPH·and OH·were especially significant.A-SPS had better effect among different Saposhnikovia polysaccharides,and the scavenging rate of DPPH·and OH·could approach to 70% when the experimental concentration was 8 mg/ml.Conclusion: A-SPS,potential main active ingredient of Saposhnikovia polysaccharide,has strong antioxidant activity and can be applied as a potential natural antioxidant in food and medicine industry.更多还原