【摘要】 目的:研究知母宁对慢性低氧高二氧化碳大鼠血栓素A2(TXA2)、前列环素(PGI2)、肺动脉压力及肺血管结构重建的影响。方法:36只SD大鼠随机分为正常对照组,肺动脉高压模型组和知母宁组,每组12只。后2组大鼠每天在低氧(8.5%~11%)高CO2(5.5%~6.5%)环境中放置8 h,每周6 d,连续4周。知母宁组大鼠每次缺O2前0.5 h腹腔内注射知母宁3.3 mg.kg-1。末次给药后次日,测定血浆TXA2和PGI2的稳定的代谢产物TXB2和6-keto-PGF1α的浓度,测定肺动脉平均压(mPAP),并观察肺细小动脉显微结构。结果:知母宁组mPAP显著低于模型组(P<0.01),肺细小动脉显微结构改变较模型组也显著降低(P<0.01)。知母宁组血浆TXB2和TXB2/6-keto-PGF1α比值均显著低于模型组(P<0.01),但6-keto-PGF1α含量则无显著改变(P>0.05)。结论:知母宁对低氧高二氧化碳致肺动脉高压大鼠具有明显的保护作用,其作用机制可能与抑制TXA2合成、减轻TXA2/PGI2的平衡失调有关。更多还原

【Abstract】 Objective:To determine the effect of chimonin on pulmonary arterial pressure,pulmonary vessel remodeling,thromboxane A2 and prostacyclin after chronic hypoxia/hypercapnia in rats.Methods: Thirty-six Sprague-Dawley rats were randomly divided into three groups.Rats were subjected to 8h of hypoxic(inspired O2 fraction=8.5%～11%) or hypercapnic(inspired CO2 fraction=5.5%～6.5%) ventilation for 4 weeks(6 days in one week).Chimonin(3.3mg·kg-1) was intraperitoneally injected 30 min before hypoxic ventilation.Following the last dose,plasma thromboxane B2(TXB2) and 6-keto-PGF1α(the stable metabolites of thromboxane A2 and prostacyclin),mean pulmonary artery pressure(mPAP),and pulmonary arteriole micromorphometric index were measured.Results: After chronic hypoxia/hypercapnia,chimonin markedly reduced the increased mPAP,pulmonary arteriole micromorphometric index [vessel wall area/total area(WA/TA)],density of cell nucleus of medial smooth muscle cell(SMC),and the thickness medial smooth muscle cell layer(PAMT,P<0.01).Chimonin also reduced the increased plasma level of TXB2 and the ratio of TXB2/6-keto-PGF1α,but did not affect the level of 6-keto-PGF1α.Conclusions: Chimonin can prevent pulmonary artery hypertension and pulmonary vessel remodeling induced by chronic hypoxia/hypercapnia in rats,which may be due to inhibiting the synthesis of TXA2 and maintaining the balance of TXA2/PGI2.更多还原