【摘要】 目的:研究玉郎伞(YLS)多糖对大鼠局灶性脑缺血再灌注损伤的保护作用,并初步探讨其作用机制。方法:W istar雄性大鼠灌胃YLS多糖高、中、低剂量(0.6,0.3,0.15 g.kg-1.d-1),连续7 d,末次给药60 m in后采用线栓法制作大鼠局灶性脑缺血再灌注模型,缺血2 h再灌注24 h后对大鼠神经功能进行评分,并测定脑梗死体积、脑含水量、脑组织中白细胞介素-β(IL-β)和肿瘤坏死因子-α(TNF-α)的含量。实验同时设尼莫地平(0.02 g.kg-1.d-1)阳性对照组和假手术组,每组10只。结果:与脑缺血再灌注模型组相比,YLS多糖能够改善大鼠的神经功能障碍,降低脑梗死体积和脑含水量,降低脑组织中的IL-1β和TNF-α含量。结论:玉郎伞多糖对大鼠急性脑缺血再灌注损伤具有保护作用,其机制可能与降低脑组织中的IL-1β和TNF-α含量有关。更多还原

【Abstract】 Objective: To investigate the protection by Yulangsan(YLS) polysaccharide against focal cerebral ischemia-reperfusion(I/R) injury in rats and the possible mechanism.Methods: In male Wistar rats,YLS polysaccharide(0.6,0.3 and 0.15g·kg-1·d-1) was intragastrically administered for 7 days.Focal cerebral ischemia was induced at 60min after last dose by middle cerebral artery occlusion(MCAO) with the method of intraluminal thread.After ischemia for 2h and reperfusion for 24h,the neurological deficit score,brain water content,infarct volume,and the changes in concentrations of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in brain tissue were determined.Sham-operation was used as the negative control and nimodipine(0.02g·kg-1·d-1) as the positive control.Results: As compared with I/R rats,YLS polysaccharide obviously reduced the neurological deficit score,brain water content,infract volume,and the concentrations of TNF-α and IL-1β in the brain tissue(P<0.01 or P<0.05).Conclusion: YLS polysaccharide has a protective effect on cerebral ischemia-reperfusion injury,and its effect may be duo to decreasing the content of TNF-α and IL-1β in the brain.更多还原