【摘要】 目的:考察非离子表面活性剂对山楂叶总黄酮中主要活性成分牡荆素-2″-O-鼠李糖苷在大鼠肠道的吸收促进作用。方法:配制含表面活性剂聚山梨酯-80(吐温-80)、聚氧乙烯氢化蓖麻油(RH40)和泊洛沙姆(F68)的山楂叶总黄酮溶液,分别在大鼠肠道上段(十二指肠和空肠)和下段(回肠和结肠)循环灌流,采用HPLC法测定溶液中牡荆素-2″-O-鼠李糖苷的浓度,计算吸收率。结果:加入3种表面活性剂后,牡荆素-2″-O-鼠李糖苷在大鼠肠内的吸收均显著增加(P<0.05),比不加表面活性剂时提高50%以上。且随表面活性剂的浓度从0.01%提高到0.1%,牡荆素-2″-O-鼠李糖苷在大鼠肠道上段和下段的吸收均显著增加。在表面活性剂的浓度较低(0.01%)时,吐温-80对药物的吸收促进作用强于RH40和F68。结论:表面活性剂吐温-80,RH40和F68均可促进山楂叶总黄酮中牡荆素-2″-O-鼠李糖在大鼠肠道上段和下段的吸收。更多还原

【Abstract】 Objective:To investigate the enhancing effect of some non-ionic surfactants on intestinal absorption of the main active components vitexin-2″-O-rhamnoside in Hawthorn Leaf total flavonoids.Methods: Solutions of Hawthorn Leaf total flavonoids were prepared by adding Tween-80,RH40 and F68,then were recirculated in rat’s upper and lower intestine,respectively.HPLC was used to determine the amounts of vitexin-2″-O-rhamnoside before and after recirculation.Results: Surfactants significantly increased absorption of vitexin-2″-O-rhamnoside(P<0.05).When the concentration of surfactants was increased from 0.01% to 0.1%,the absorption of vitexin-2″-O-rhamnoside was enhanced both in rat’s upper and in lower intestine.Tween-80 possessed the much bigger absorption enhancement than RH40 and F68 at the low concentration of surfactants(0.01%).Conclusion: These surfactants,Tween-80,RH40 and F68,can promote absorption of vitexin-2″-O-rhamnoside in rat’s upper and lower intestine.更多还原