【摘要】 目的探讨S100A4蛋白在人非小细胞肺癌(NSCLC)间质中的表达及其临床意义,以确定其与肿瘤的侵袭、转移及预后的关系。方法130例NSCLC患者手术切除的肺癌组织标本,应用免疫组织化学方法(SP法)检测S100A4蛋白在肺癌组织间质中的表达,分析其与预后的关系。结果S100A4蛋白在NSCLC间质表达的总阳性率为72.3%(94/130),在鳞状细胞癌、腺癌、腺鳞癌和大细胞癌间质表达的阳性率分别为84.3%、59.6%、70.0%和75.0%。S100A4蛋白在NSCLC间质的阳性表达与淋巴结转移(χ2=18.91,P=0.000)、远处转移(χ2=5.51,P=0.019)及TNM分期(χ2=21.54,P=0.000)明显相关。间质S100A4蛋白表达阳性患者3年生存率为36.2%(34/94),明显低于表达阴性的患者[63.9%(23/36),P=0.003]。Cox风险比例模型分析结果表明,年龄≤50岁(OR=1.866)、有淋巴结转移(OR=1.826)、有远处转移(OR=6.224)、肿瘤低、未分化(OR=1.793)、TNM分期Ⅲ～Ⅳ期(OR=2.573)和间质中S100A4蛋白表达阳性(OR=1.776)是影响肺癌患者预后的危险因素。结论S100A4蛋白在NSCLC间质中的表达与肺癌的侵袭、转移、分期及预后密切相关,它有希望成为一种能预测肿瘤进展及指导临床治疗的标记物。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of S100A4 protein in tumor-stroma of non-small cell lung cancer(NSCLC) to study its correlation with invasion, metastasis and prognosis. Methods Immunohistochemical staining(S-P method)for S100A4 protein expression was performed in tissue sections from 130 patients with NSCLC operated and to analyze association of S100A4 protein with clinicopathological parameters in lung cancer and prognosis. Results The total positive expression rates of S100A4 protein in stroma of NSCLC was 72.3%. The positive expression rates of S100A4 protein in stroma of squamous cell carcinoma, adenocarcinoma, adenosquamous carcinoma and large cell lung cancer were 84.3%,59.6%,70.0% and 75% respectively.The expression of S100A4 protein was significantly associated with lymph node metastasis (χ2=18.91, P=0.000), distant metastasis(χ2=5.51, P=0.019) and TNM stage (χ2=21.54, P=0.000). The 3 years survival rates of patients whose tumour-stroma stained positive for S100A4 was lower than that of patients whose tumour-stroma stained negative (36.2% vs. 63.9%, P=0.003). Cox’ risk ratio model analysis indicated that age ≤50 years (OR=1.866), lymph node metastasis(OR=1.826), distant metastasis(OR=6.224), lower histology differentiation and undifferentiation (OR=1.793), TNM stage Ⅲ-Ⅳ (OR=2.573) and positive expression of S100A4 protein in stroma of NSCLC(OR=1.776) were significantly independent prognostic factors which affected survival. Conclusion Expression of S100A4 protein in stroma of NSCLC is significantly associated with invasion, metastasis, TNM stage and prognosis. S100A4 protein might become a marker for prediction of tumor progression of disease and clinical therapy.更多还原