【摘要】 目的观察卒中高危人群血浆组织型纤溶酶原激活物(t-PA)活性的变化,从而了解卒中高危人群凝血与纤溶功能状况,为以益气活血法预防卒中临床方案的实施提供依据。方法选择本院门诊就诊的患者,按诊断和治疗分为卒中高危组、治疗组;选择同期健康体检者作为正常对照组。治疗组在治疗前及治疗后,正常对照组在查体时各采一次空腹肘静脉血,卒中高危组实验前后各采血一次,采用发色底物法测定血浆中(t-PA)活性。结果正常对照组(50例)血浆t-PA活性〔(0.576±0.052)kU/L〕>治疗组〔51例,(0.513±0.066)kU/L〕>卒中高危组〔51例,(0.411±0.078)kU/L〕。统计学分析显示:正常对照组显著高于卒中高危组(P<0.05),治疗组显著高于卒中高危组,但仍低于正常对照组(P均<0.05)。结论①有卒中先兆症状的人群血浆t-PA活性普遍降低;②血浆t-PA活性对卒中高危人群具有预测价值;③益气活血疗法有利于活化血管内皮细胞的分泌功能,提高人体血浆中t-PA的活性。更多还原

【Abstract】 Objective To observe the changes of activity of plasma tissue plasminogen activator(t-PA) in a crowd of people with stroke at high risk in order to realize the functional status of blood coagulation and fibrinolysis in the crowd and provide a clinical practical basis for prevention of stroke with traditional Chinese medicine therapy of benefiting Qi and promoting blood(益气活血疗法).Methods According to the diagnosis and treatment,people coming to out-patient clinic were divided into two groups: stroke with high risk group(n=50) and treatment group(n=51);meanwhile,50 cases with health check-up were elected as normal control group.Blood samples collected from ulnar vein at empty stomach were adopted respectively,collected once before and after treatment in treatment group,once at health check-up in normal control group,and once before and after experiment in stroke with high risk group.The activity of plasma t-PA was determined by enzyme assay equipment with luminescence substrate method.Results The plasma activity of t-PA in treatment group((0.513±0.066) kU/L) was lower than that in normal control group((0.576±0.052) kU/L) and higher than that in stroke with high risk group((0.411±0.078) kU/L).The results of comparison in the three groups were that the activity of t-PA in normal control group and treatment group were markedly higher than that in stroke with high risk group,but the activity of t-PA in treatment group was still significantly lower than that in normal control group(both P<0.05).Conclusion ① Generally,the plasma activity of t-PA decreases in a crowd with stroke pre-symptom.②The plasma activity of t-PA has prognostic value in a crowd with stroke at high risk.③ Benefiting Qi and promoting blood therapy is beneficial to the activation of the vascular endothelial cells to secrete and raise the plasma activity of t-PA in human body.更多还原