【摘要】 目的探讨阴虚内热证系统性红斑狼疮(SLE)患者用滋阴清热方等治疗前、中(6周)、后(12周)外周血淋巴细胞(PBMC)蛋白表达谱的动态变化。方法应用化学蛋白芯片及表面增强激光解吸离子化(SELDI)方法建立15例女性阴虚内热证SLE患者外周血淋巴细胞蛋白表达谱,用ZUCI-PDAS软件包分析蛋白表达差异,采用基因数据库与ExPASy蛋白数据库交连比对法初步确定质荷峰蛋白属性。结果发现20个阴虚内热证SLE患者治疗前后差异有统计学意义的蛋白峰,建立的四个蛋白质峰(4787、2724、4804、4831)判别模式可以对SLE样品是治疗前还是治疗3个月后进行判别,预测治疗前与治疗3个月后的正确率分别为100%与90%。结论阴虚内热证SLE治疗前、中、后存在淋巴细胞蛋白表达模式的差异,提示滋阴清热方等治疗可能通过调节淋巴细胞多种蛋白的表达而起到治疗作用。进一步深入研究有望找到治疗相关蛋白。更多还原

【Abstract】 Objective To investigate the dynamic changes of protein expression of peripheral blood mononuclear cell(PBMC) of systemic lupus erythematosus(SLE) patients with internal heat due to Yin deficiency(IHDYD) according to three different stages: before cure,6 weeks and 12 weeks treating with nourishing Yin clearing heat formula(NYCHF). Methods Surface-enhanced laser desorption/ionization (SELDI) method was used to protein build expression of PBMC of 15 female IHDYD SLE patients with the score between 5 to 25 according to SLEDAI system of the United States and Canada SLE center,and ZUCI-PDAS software package was used to analyze protein expressing difference,adopt the gene data base and the ExPASy to analyze the character of protein mark. Results After 12 weeks,twenty protein markers had significant differences. Discrimination mode with four markers(4787,2724,4804,4831) could discern samples of prior treatment or after 12 weeks’ treatment with a exactitude rate as 100% and 90%. Conclusion Differences of protein expression were found before,during and after the treatment. NYCHF may cure IHDYD SLE patients by regulating protein expression of PBMC. Further study may find treatment-related protein.更多还原