【摘要】 目的研究锌离子(Zn2+)对阿尔茨海默病(AD)大鼠海马微管相关蛋白-2(MAP-2)表达的影响。方法大鼠60只分4组:正常组、模型组和AD前补锌组、AD后补锌组。采用β-淀粉样肽(amyloid,Aβ)大鼠侧脑室注射制作AD动物模型,行为学检测和大鼠海马组织电镜切片观察检测动物模型。用免疫荧光染色和Westernblot法检测大鼠海马组织MAP-2的表达。结果Aβ脑室内灌注造成Aβ沉积的AD模型在行为学和病理改变上一定程度地模拟了AD。模型组和AD后补锌组大鼠海马区MAP-2蛋白表达与正常组大鼠相比显著下降,MAP-2蛋白荧光检测发现模型组和AD后补锌组大鼠海马区MAP-2阳性神经元数量明显减少,AD前补锌组大鼠海马区MAP-2蛋白表达和MAP-2阳性神经元数量与正常组相比差异无显著性。结论在AD形成期间适量补锌可显著改善海马受损神经元细胞结构和细胞骨架蛋白的结构及含量,而在AD形成之后,补锌已不能引起细胞结构的改善。更多还原

【Abstract】 [Objective] To study the influence of Zn2+ over the expression of microtubule-associated protein-2 (MAP-2) of rats with Alzheimer disease (AD rats). [Methods] The rats were divided into 4 groups: normal group, model group, zinc reinforce pro-AD group and zinc reinforce post-AD group. Beta-amyloid protein was injected into the lateral ventricle of rats′ brain to make AD animal model, then detected the behaviour of the rats according to the theory of ethology,observed and detected the rat′s hippocamp tissue under electron microscopic section and tested the MAP-2 expression of rat′s hippocamp tissue by means of immunofluorescence stain and Western blot. [Results] The AD rat model made by filling Beta-amyloid protein into the lateral ventricle imitated the AD in ethology and pathology to some extent. The MAP-2 expression in the hippocamp of model group and zinc reinforce post-AD group decreased greatly in contrast to the normal group. It was found by means of protein fluorescence detection that the number of MAP-2 masculine neurone in the hippocamp of AD rat in model group and zinc reinforce post-AD group decreased distinctly. There was not distinct difference in the MAP-2 expression and the number of MAP-2 masculine neurone between normal group and zinc reinforce pro-AD group. [Conclusion] Appropriate zinc reinforce during the formation of AD can improve the structure of the damaged neurone cell as well as the structure and amount of cytoskeletal protein, while, after the formation of AD, zinc reinforce can not improve the structure of the damaged cell.更多还原